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Titulo:	Ectopic expression of new alternative splice variant of Smac/DIABLO increases mammospheres formation
Autores:	MARTINEZ-RUIZ GU VICTORIA-ACOSTA G VAZQUEZ-SANTILLAN KI JIMENEZ-HERNANDEZ L MUNOZ-GALINDO L GISELA CEBALLOS CANCINO VILMA ARACELI MALDONADO LAGUNAS JORGE MELÉNDEZ ZAJGLA
Tipo de Licencia:	http://creativecommons.org/licenses/by-nc/4.0
Fecha de Publicación:	15 de Agosto de 2014
Resumen:	Smac-alpha is a mitochondrial protein that, during apoptosis, is translocated to the cytoplasm, where it negatively regulates members of the inhibitor of apoptosis (IAP) family via the IAP-binding motif (IBM) contained within its amino-terminus. Here, we describe a new alternative splice variant from Smac gene, which we have named Smac-epsilon. Smac-epsilon lacks both an IBM and a mitochondrial-targeting signal (MTS) element. Smac-epsilon mRNA exhibits a tissue-specific expression pattern in healthy human tissues as well as in several cancer cell lines. The steady-state levels of endogenous Smac-epsilon protein is regulated by the proteasomal pathway. When ectopically expressed, this isoform presents a cytosolic localization and is unable to associate with or to regulate the expression of X-linked Inhibitor of apoptosis protein, the best-studied member of IAP family. Nevertheless, over-expression of Smac-epsilon increases mammosphere formation. Whole genome expression analyses from these mammospheres show activation of several pro-survival and growth pathways, including Estrogen-Receptor signaling. In conclusion, our results support the functionality of this new Smac isoform.
Editorial:	International Journal of Clinical and Experimental Pathology
Idioma:	Ingles
Palabras Clave:	Breast Neoplasms/genetics/metabolism/pathology Cytosol/metabolism Female Gene Expression Profiling/methods Gene Expression Regulation Neoplastic Gene Regulatory Networks HeLa Cells Humans Intracellular Signaling Peptides and Proteins/genetics/metabolism MCF-7 Cells Mitochondrial Proteins/genetics/*metabolism Oligonucleotide Array Sequence Analysis Proteasome Endopeptidase Complex/metabolism Protein Isoforms Proteolysis Spheroids Cellular Transcription Genetic Transfection Up-Regulation
URL:	https://repositorio.inmegen.gob.mx/record/325

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