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Titulo:	Microarray comparative genomic hybridization detection of chromosomal imbalances in uterine cervix carcinoma
Autores:	ALFREDO HIDALGO MIRANDA BAUDIS M PETERSEN I ARREOLA H PINA P VAZQUEZ-ORTIZ G HERNANDEZ D GONZALEZ J LAZOS M LOPEZ R PEREZ C GARCIA J VAZQUEZ K ALATORRE B SALCEDO M
Tipo de Licencia:	http://creativecommons.org/licenses/by/4.0
Fecha de Publicación:	9 de Julio de 2005
Resumen:	BACKGROUND: Chromosomal Comparative Genomic Hybridization (CGH) has been applied to all stages of cervical carcinoma progression, defining a specific pattern of chromosomal imbalances in this tumor. However, given its limited spatial resolution, chromosomal CGH has offered only general information regarding the possible genetic targets of DNA copy number changes. METHODS: In order to further define specific DNA copy number changes in cervical cancer, we analyzed 20 cervical samples (3 pre-malignant lesions, 10 invasive tumors, and 7 cell lines), using the GenoSensor microarray CGH system to define particular genetic targets that suffer copy number changes. RESULTS: The most common DNA gains detected by array CGH in the invasive samples were located at the RBP1-RBP2 (3q21-q22) genes, the sub-telomeric clone C84C11/T3 (5ptel), D5S23 (5p15.2) and the DAB2 gene (5p13) in 58.8% of the samples. The most common losses were found at the FHIT gene (3p14.2) in 47% of the samples, followed by deletions at D8S504 (8p23.3), CTDP1-SHGC- 145820 (18qtel), KIT (4q11-q12), D1S427-FAF1 (1p32.3), D9S325 (9qtel), EIF4E (eukaryotic translation initiation factor 4E, 4q24), RB1 (13q14), and DXS7132 (Xq12) present in 5/17 (29.4%) of the samples. CONCLUSION: Our results confirm the presence of a specific pattern of chromosomal imbalances in cervical carcinoma and define specific targets that are suffering DNA copy number changes in this neoplasm.
Editorial:	BMC CANCER
Idioma:	Ingles
Palabras Clave:	Cell Line Tumor Chromosome Aberrations Chromosome Deletion DNA/genetics DNA Primers/chemistry DNA Neoplasm Female Gene Expression Regulation Neoplastic HeLa Cells Humans Image Processing Computer-Assisted Loss of Heterozygosity Nucleic Acid Hybridization Oligonucleotide Array Sequence Analysis/methods Papillomaviridae/genetics Polymerase Chain Reaction Uterine Cervical Neoplasms/diagnosis/*genetics/metabolism
URL:	https://repositorio.inmegen.gob.mx/record/40

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